Journal: European journal of histochemistry : EJH

Article Title: Sodium hyaluronate promotes proliferation, autophagy, and migration of corneal epithelial cells by downregulating miR-18a in the course of corneal epithelial injury.

doi: 10.4081/ejh.2023.3663

Figure Lengend Snippet: Figure 5. SH induced autophagy and cell migration by downregulating miR-18a in CEI model cells. CEI model cells were treated with SH and miR-18a mimics, respectively. A) Cell migration was analyzed by the Transwell assay, and the numbers of migrated cells in each group were counted. B) Western blotting showed changes in LC3B, Beclin1, and P62 expression. C) LC3B expression was examined by IF staining. Magnification: x200.

Article Snippet: The primary antibodies used were Col1A1 (BA0325, 1:800; Boster), FN (A00564-1, 1:1000; Boster), LC3B (ab192890, 1:2000; Abcam), Beclin1 (BA3123-2, 1:1500; Boster), P62 (BA2849, 1:1000; Boster), and GAPDH (ab8245, 1:5000; Abcam).

Techniques: Migration, Transwell Assay, Western Blot, Expressing, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Dioscin ameliorates peritoneal fibrosis by inhibiting epithelial-to-mesenchymal transition of human peritoneal mesothelial cells via the TLR4/MyD88/NF-κB signaling pathway

doi:

Figure Lengend Snippet: Dioscin inhibits LPS-induced fibrosis in HMrSV5 cells. Western blotting for detecting protein levels of α-SMA, collagen I and fibronectin. ###P < 0.001 compared with negative control group or dioscin (1.0 μg/ml) group. ***P < 0.001 compared with LPS group. NC, negative control; LPS, lipopolysaccharide; Dio, Dioscin; α-SMA, α-smooth muscle actin.

Article Snippet: Then the membranes were incubated with following primary antibodies: α-SMA (1:100, Proteintech Group), collagen I (1:100, Proteintech Group), fibronectin (1:250, Abcam), TLR4 (1:1000, Proteintech Group), MyD88 (1:1000, Proteintech Group), NF-κB (1:1000, Proteintech Group), TGF-β1 (1:1000, Proteintech Group), p-Smad2/Smad2 (1:1000, Proteintech Group), respectively.

Techniques: Western Blot, Negative Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Dioscin ameliorates peritoneal fibrosis by inhibiting epithelial-to-mesenchymal transition of human peritoneal mesothelial cells via the TLR4/MyD88/NF-κB signaling pathway

doi:

Figure Lengend Snippet: Dioscin inhibits EMT and fibrosis through TLR4/MyD88/NF-κB pathway in HMrSV5 cells. A. Western blotting for assessing protein levels of TLR4, MyD88, NF-κB, TGF-β1, p-Smad2, Smad2, α-SMA, collagen I and fibronectin. B. immunofluorescence assay for detecting expressions of α-SMA, collagen I and fibronectin in LPS+TLR4 group and LPS+TLR4+dioscin (0.5 μg/ml) group. ###P < 0.001 compared with negative control group or Ppicza group. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with LPS+ pPICZA group or LPS+TLR4 group. NC, negative control; LPS, lipopolysaccharide; Dio, Dioscin; α-SMA, α-smooth muscle actin; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor κB; TGF-β1, transforming growth factor-β1; TLR4, Toll-like receptor (TLR) 4.

Article Snippet: Then the membranes were incubated with following primary antibodies: α-SMA (1:100, Proteintech Group), collagen I (1:100, Proteintech Group), fibronectin (1:250, Abcam), TLR4 (1:1000, Proteintech Group), MyD88 (1:1000, Proteintech Group), NF-κB (1:1000, Proteintech Group), TGF-β1 (1:1000, Proteintech Group), p-Smad2/Smad2 (1:1000, Proteintech Group), respectively.

Techniques: Western Blot, Immunofluorescence, Negative Control

Journal: Molecular Medicine Reports

Article Title: iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy

doi: 10.3892/mmr.2020.11472

Figure Lengend Snippet: Differentially expressed proteins identified between -Treatment samples and control samples using isobaric tags for relative and absolute quantitation technology.

Article Snippet: Serum concentrations of keratin 1 (KRT1; cat. no. KTE61888; Abbkine Scientific Co., Ltd.), apolipoprotein B (APOB; cat. no. KA1028; Abnova), fibronectin 1 (FN1; cat. no. KA3039; Abnova), antithrombin III (SERPINC1; cat. no. KA5093; Abnova) and α-1-antitrypsin (SERPINA1; cat. no. KA0459; Abnova) of the 25 samples in each group were determined according to the manufacturers' protocols from the ELISA kits.

Techniques: Control, Quantitation Assay

Journal: Molecular Medicine Reports

Article Title: iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy

doi: 10.3892/mmr.2020.11472

Figure Lengend Snippet: Differentially expressed proteins identified between +Treatment samples and control samples using isobaric tags for relative and absolute quantitation technology.

Article Snippet: Serum concentrations of keratin 1 (KRT1; cat. no. KTE61888; Abbkine Scientific Co., Ltd.), apolipoprotein B (APOB; cat. no. KA1028; Abnova), fibronectin 1 (FN1; cat. no. KA3039; Abnova), antithrombin III (SERPINC1; cat. no. KA5093; Abnova) and α-1-antitrypsin (SERPINA1; cat. no. KA0459; Abnova) of the 25 samples in each group were determined according to the manufacturers' protocols from the ELISA kits.

Techniques: Control, Quantitation Assay

Journal: Molecular Medicine Reports

Article Title: iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy

doi: 10.3892/mmr.2020.11472

Figure Lengend Snippet: Validation of proteomics results by western blot analysis. (A) Western blot analysis was used for the validation of 5 proteins (KRT1, APOB, FN1, SERPINC1 and SERPINA1) from control, -Treatment and +Treatment samples; β-actin was used as an internal control. (B) Histogram of western blot analysis. The control group is shown in gray, the -Treatment group is blue and the +Treatment group is brown. Data are expressed as mean ± standard deviation (n=25 biological replicates in each group). The multiple comparisons test of control vs. -Treatment and control vs. +Treatment are performed using analysis of variance with Dunnett's test. *P<0.05, **P<0.01 and ***P<0.001 vs. control. KRT1, keratin 1; APOB, apolipoprotein B; FN1, fibronectin 1; SERPINC1, antithrombin III; SERPINA1, α-1-antitrypsin.

Article Snippet: Serum concentrations of keratin 1 (KRT1; cat. no. KTE61888; Abbkine Scientific Co., Ltd.), apolipoprotein B (APOB; cat. no. KA1028; Abnova), fibronectin 1 (FN1; cat. no. KA3039; Abnova), antithrombin III (SERPINC1; cat. no. KA5093; Abnova) and α-1-antitrypsin (SERPINA1; cat. no. KA0459; Abnova) of the 25 samples in each group were determined according to the manufacturers' protocols from the ELISA kits.

Techniques: Western Blot, Control, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: iTRAQ-based proteomic analysis reveals potential regulatory networks in dust mite-related asthma treated with subcutaneous allergen immunotherapy

doi: 10.3892/mmr.2020.11472

Figure Lengend Snippet: Validation of the 5 candidate proteins (KRT1, APOB, FN1, SERPINC1 and SERPINA1) by ELISA in the control children, and -Treatment and +Treatment patients. The control group is shown in gray, the -Treatment in blue and the +Treatment in brown. Data are expressed as mean ± standard deviation (n=25 biological replicates in each group). The multiple comparisons test of control vs. -Treatment and control vs. +Treatment are performed using ANOVA with Dunnett or Welch with Dunnett's T3 test based on the result of variance homogeneity. Except for KRT1 (ANOVA statistics), the variances of APOB, FN1, SERPINC1 and SERPINA1 were not homogenous. Δ Data were analyzed using a Welch test. *P<0.05 and ***P<0.001 vs. control. KRT1, keratin 1; APOB, apolipoprotein B; FN1, fibronectin 1; SERPINC1, antithrombin III; SERPINA1, α-1-antitrypsin; ANOVA, analysis of variance.

Article Snippet: Serum concentrations of keratin 1 (KRT1; cat. no. KTE61888; Abbkine Scientific Co., Ltd.), apolipoprotein B (APOB; cat. no. KA1028; Abnova), fibronectin 1 (FN1; cat. no. KA3039; Abnova), antithrombin III (SERPINC1; cat. no. KA5093; Abnova) and α-1-antitrypsin (SERPINA1; cat. no. KA0459; Abnova) of the 25 samples in each group were determined according to the manufacturers' protocols from the ELISA kits.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Standard Deviation